Chinese Hamster Ovary (CHO) cells are used in the development and production of recombinant proteins.
TLA analyses are used to sequence:
- The genomic position(s) of transgene integration site(s).
- Single Nucleotide Variants in the transgene sequence.
- Structural changes in the transgene sequence.
- Changes resulting from targeted (trans)gene editing.
The TLA technology can be utilised in various stages of CHO cell line development such as in early stage clone selection, or to assess the genetic stability and clonality of Master Cell Banks, Working Cell Banks and End-of Production cells.
Cergentis supports many leading pharmaceutical companies in CHO cell development and Quality Control.
Webinar CHO transgene and integration site sequencing
See below to watch a replay of a recent webinar on the complete genetic characterisation of transgenes, integration sites and gene editing events in CHO cell lines.
"TLA analysis has proven to be very useful to quality control genetically engineered cell lines”John Wiseman Ph.D.(AstraZeneca, Mölndal, Sweden)
"TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”Nik Klymiuk Ph.D.(Faculty Veterinary Science Ludwig Maximilians University Munich, Germany)
"Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”Prof. Joshua Sanes(Harvard University, Department of Molecular and Cellular Biology, USA)
"TLA outperforms traditional methods used in CHO cell line characterisation and provides higher resolution and sensitivity.”Stefanie Bartels Ph.D.(Synthon, the Netherlands)
"TLA is a highly efficient method for mapping the insertion site of any transgene.”Søren Warming Ph.D.(Senior Scientist Genentech, San Francisco, USA)
- Application notes
Application note on the use of the TLA technology in transgene and integration site sequencing in CHO cell lines.