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Transgenes & Integration sites

TLA analyses with both the cell-based and genomic DNA protocols enable the sequencing of integration sites and integrated sequences in a wide variety of applications.

 

Examples include: 

 

  • Transgene & Integration site sequencing in cell-lines
  • Transgene & Integration site sequencing in animal models
  • Transgene & integration site sequencing in plants  
  • HIV virus and integration site sequencing in humans 
  • HBV virus integration site sequencing in tumours

 

TLA analyses are used to sequence:

 

  • The genomic position(s) of integration site(s).
  • Single Nucleotide Variants in the transgene sequence.
  • Structural changes in the transgene sequence.
  • Changes resulting from targeted (trans)gene editing.

 

 

Webinar transgene and integration site sequencing

See below to watch a replay of a recent webinar on the complete genetic characterisation of transgenes, integration sites and gene editing events.

 

 

 

 

  • "TLA analysis has proven to be very useful to quality control genetically engineered cell lines”

    John Wiseman Ph.D.(AstraZeneca, Mölndal, Sweden)
  • "TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”

    Nik Klymiuk Ph.D.(Faculty Veterinary Science Ludwig Maximilians University Munich, Germany)
  • "Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”

    Prof. Joshua Sanes(Harvard University, Department of Molecular and Cellular Biology, USA)
  • "TLA outperforms traditional methods used in CHO cell line characterisation and provides higher resolution and sensitivity.”

    Stefanie Bartels Ph.D.(Synthon, the Netherlands)
  • "TLA is a highly efficient method for mapping the insertion site of any transgene.”

    Søren Warming Ph.D.(Senior Scientist Genentech, San Francisco, USA)
  •  

    Blog:

    "The main advantage of TLA over conventional approaches is that it generates complete sequence information of a region of interest."

    Taconic
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