Gene Sequencing & Haplotyping
Whereas conventional approaches for targeted sequencing are hypothesis-driven, largely miss intronic genetic variation, and will fail to identify a large share of structural variants, the TLA technology enables hypothesis-neutral complete sequencing of any genomic locus.
TLA thus enables the identification of all genetic variation in loci of interest.
In addition, TLA enables haplotyping across large physical distances, both with short- and long-read sequencing technologies.
The TLA technology is highly flexible. Any genomic region of interest can be sequenced by simply using one primer pair complementary to a short locus-specific sequence.
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Blog:
Illumina
"Targeted Locus Amplification (TLA), a comprehensive method of selectively amplifying genes and detecting all variation within, while preserving haplotype information.” -
"Unlike other targeted sequencing methods, TLA works without detailed prior locus information, as one primer pair is sufficient to amplify and sequence tens to hundreds of kilobases of surrounding DNA.”
Pacific Biosciences
- Application notes
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Application note on the TLA protocol for isolated genomic DNA. -
Application Note Pacific Biosciences - Targeted Sequencing and Chromosomal Haplotype Assembly Using Cergentis TLA Technology with PacBio SMRT Sequencing. - Publications
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