CHO cells

Chinese Hamster Ovary (CHO) cells are used in the development and production of recombinant proteins via genome modification.


TLA analyses identify:


  • The genomic position(s) of transgene integration site(s).
  • Single nucleotide variants in the transgene sequence.
  • Structural changes in the transgene sequence and integration site.
  • Changes resulting from targeted (trans)gene editing.


The TLA technology can be utilised in various stages of CHO cell line development (e.g. early stage clone selection) or to assess the genetic stability and clonality of Master Cell Banks, Working Cell Banks and End-of-Production cells.


Cergentis supports many leading pharmaceutical companies in CHO cell development and Quality Control.



Watch a replay of a recent webinar on TLA-based targeted transgene & integration site sequencing in (CHO) cell line development and quality control.






  • "TLA analysis has proven to be very useful to quality control genetically engineered cell lines”

    John Wiseman Ph.D.(AstraZeneca, Mölndal, Sweden)
  • "TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”

    Nik Klymiuk Ph.D.(Faculty Veterinary Science Ludwig Maximilians University Munich, Germany)
  • "Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”

    Prof. Joshua Sanes(Harvard University, Department of Molecular and Cellular Biology, USA)
  • "TLA outperforms traditional methods used in CHO cell line characterisation and provides higher resolution and sensitivity.”

    Stefanie Bartels Ph.D.(Synthon, the Netherlands)
  • "TLA is a highly efficient method for mapping the insertion site of any transgene.”

    Søren Warming Ph.D.(Senior Scientist Genentech, San Francisco, USA)
© 2012-2018 Cergentis B.V. All rights reserved.
For Research Use Only.Not for use in diagnostic procedures.
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