CHO Cell Line Development
Chinese Hamster Ovary (CHO) cells are used in the development and production of recombinant proteins.
TLA analyses can identify:
- The genomic position(s) of transgene integration site(s).
- Single nucleotide variants in the transgene sequence.
- Structural changes in the transgene sequence and integration site.
- Changes resulting from targeted (trans)gene editing.
The TLA technology can be utilised in various stages of CHO cell line development (e.g. early stage clone selection) or to assess the genetic stability and clonality of Master Cell Banks, Working Cell Banks and End-of-Production cells.
Cergentis supports many leading pharmaceutical companies in CHO cell development and Quality Control.
Watch a replay of a recent webinar on TLA-based targeted transgene & integration site sequencing in (CHO) cell line development and quality control.
"TLA analysis has proven to be very useful to quality control genetically engineered cell lines”
Dr. John Wiseman - Associate Director (Molndal, Sweden)
"TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”
Ludwig Maximilians University Munich
Dr. Nik Klymiuk - Molecular Animal Breeding and Biotechnology (Germany)
"TLA outperforms traditional methods used in CHO cell-line characterisation and provides higher resolution and sensitivity.”
Dr. Stefanie Bartels - Project Leader - Cell Line Development (The Netherlands)
"TLA is a highly efficient method for mapping the insertion site of any transgene.”
Dr. Søren Warming - Associate Director/Senior Scientist (San Francisco, CA, USA)
"Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”
Prof. Dr. Joshua Sanes - Department of Molecular and Cellular Biology (Cambridge, MA, USA)
- Application notes
Application note on the use of the TLA technology in transgene and integration site sequencing in CHO cell lines.
Application note on Genedata Selector for Efficient and High Quality CHO Cell Line Development - Leveraging TLA for complete sequencing of expression constructs and integration sites
Aeschlimann SH et al. (2019)
Novartis Institutes for BioMedical Research & Cergentis
Aebischer-Gumy C et al. (2018)
Glenmark Pharmaceuticals & Thomas A. Little Consulting
Patel NA et al. (2018)
Boyd D et al. (2018)
Baumann M et al. (2017)
Austrian Centre of Industrial Biotechnology (ACIB), MilliporeSigma & University of Natural Resources and Life Sciences (BOKU)
Zhu J & Hatton D (2017)
Mickus B (Presentation - 2016)
Kaas CS et al. (2015)
Novo Nordisk & Technical University of Denmark