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CHO Cell Line Development

Chinese Hamster Ovary (CHO) cells are used in the development and production of recombinant proteins.

 

TLA analyses can identify:

 

  • The genomic position(s) of transgene integration site(s).
  • Single nucleotide variants in the transgene sequence.
  • Structural changes in the transgene sequence and integration site.
  • Changes resulting from targeted (trans)gene editing.

 

The TLA technology can be utilised in various stages of CHO cell line development (e.g. early stage clone selection) or to assess the genetic stability and clonality of Master Cell Banks, Working Cell Banks and End-of-Production cells.

 

Cergentis supports many leading pharmaceutical companies in CHO cell development and Quality Control.

 

Webinar

Watch a replay of a recent webinar on TLA-based targeted transgene & integration site sequencing in (CHO) cell line development and quality control.

 

 

 

 

 

  • "TLA analysis has proven to be very useful to quality control genetically engineered cell lines”

                                                                                       AstraZeneca                                                                                   

    Dr. John Wiseman - Associate Director (Molndal, Sweden)  

  • "TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”

    Ludwig Maximilians University Munich

    Dr. Nik Klymiuk - Molecular Animal Breeding and Biotechnology (Germany)

  • "TLA outperforms traditional methods used in CHO cell-line characterisation and provides higher resolution and sensitivity.”

                                             Synthon                                         

    Dr. Stefanie Bartels - Project Leader - Cell Line Development (The Netherlands)

  •    "TLA is a highly efficient method for mapping the insertion site of any transgene.” 

                                                                                        Genentech                                                                                        

    Dr. Søren Warming - Associate Director/Senior Scientist (San Francisco, CA, USA)

  • "Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”

                                       Harvard University                                     

    Prof. Dr. Joshua Sanes - Department of Molecular and Cellular Biology (Cambridge, MA, USA)

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