Gene editing techniques such as CRISPR-Cas9 enable the generation of both small and large genetic alterations.
TLA analyses, using primers in proximity to a targeted site, can be used to determine what sequence changes have occurred in that locus.
In addition, TLA analyses can, using transgene-specific primer pairs, be used to determine the exact sequence of desired and (un)desired integration events of any transgene sequence.
See below to watch a replay of a recent webinar on the complete genetic characterisation of transgenes, integration sites and gene editing events.
"TLA analysis has proven to be very useful to quality control genetically engineered cell lines.”John Wiseman Ph.D.(AstraZeneca, Mölndal, Sweden)
"TLA has proven to be a powerful technology for transgene integration site sequencing and for evaluation of structural changes in the transgene and/or integration site.”Nik Klymiuk Ph.D.(Faculty Veterinary Science Ludwig Maximilians University Munich, Germany)
"Cergentis quickly determined transgene insertion for three out of three lines along with providing information on deletions and rearrangements in chromosomal DNA at the site. The service was quick, accurate and reasonably priced, and reports were complete and helpful.”Prof. Joshua Sanes(Harvard University, Department of Molecular and Cellular Biology, USA)
"TLA outperforms traditional methods used in CHO cell-line characterisation and provides higher resolution and sensitivity.”Stefanie Bartels Ph.D.(Synthon, the Netherlands)
"TLA is a highly efficient method for mapping the insertion site of any transgene.”Søren Warming Ph.D.(Senior Scientist Genentech, San Francisco, USA)
- Application notes
Application note on TLA-based transgene and -integration site sequencing.
Lu Q, Livi GP, Modha S, Yusa K, Macarron R & Dow DJ.
Eyquem J et al.
Renaud J-B et al.