TLA-based targeted sequencing provides breakpoint sequences resulting from structural changes or gene fusions.
These breakpoint sequences can be the basis for personalised qPCR-based minimal residual disease tests.
Minimal residual disease (MRD) tests are used to determine prognosis and response to therapy in acute and chronic leukaemia, lymphoma and myeloma patients.
Cergentis supports the development of personalised MRD tests with its kits and services.
Examples of the use of genomic breakpoint sequences in qPCR-based minimal residual disease tests by leading European diagnostic labs are presented in the application note below.
"We use TLA to sequence gene fusions and other rearrangements in genes of interest. We have been impressed by the quality of TLA and Cergentis’ work and are testing the use of identified breakpoint sequences as targets for personalised minimal residual disease tests.”
Princess Máxima Center for Pediatric Oncology
Dr. Roland Kuiper - Senior PI (Utrecht, The Netherlands)
"Thank you so much. Data about the structural changes in our patient are very clear. Really powerful technology!”
University of Turin
Prof. Dr. Alfredo Brusco - Department of Medical Sciences (Italy)
"Targeted Locus Amplification enables fast identification of new chromosomal rearrangements and genomic breakpoint sequences in human T-ALL and unraveled a higher complexity of chromosomal translocations of known T-ALL oncogenes as thus far appreciated.”
Prof. Dr. Pieter van Vlierberghe - Center for Medical Genetics (Belgium)
- Application notes
Application note on MRD testing using TLA data
Dogliotti I & Ferrero S (2017)
University of Torino
Targeted Locus Amplification (TLA): A Novel Next Generation Sequencing (NGS) Technology to Detect New Molecular Markers and Monitoring Minimal Residual Disease (MRD) in Mantle Cell and Follicular Lymphoma
Genuardi E et al. (2017)
A.O.U. Citta della Salute e della Scienza di Torino, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Cergentis, Clinica Humanitas/Gavazzeni, University of Modena e Reggio Emilia & University of Torino