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Gene Sequencing

Whereas conventional approaches for targeted sequencing are hypothesis-driven, largely miss intronic genetic variation and will fail to identify a large share of structural variants, Cergentis' TLA technology enables hypothesis-neutral, complete sequencing of any genomic locus using a single primer pair. TLA thereby enables the identification of all genetic variations in loci of interest.


The TLA technology is highly flexible. Any genomic region of interest can be sequenced by simply using one primer pair complementary to a short locus-specific sequence.

 

In addition, TLA enables haplotyping across large physical distances, both with short- and long-read sequencing technologies.*

 

 

* Cergentis does not currently offer this as a routine service. For more information please contact sales.

  • Application notes
  • Publications
  • Webinars
  • Application notes
  • Poster: TLA & Long Read Sequencing: Efficient Targeted Sequencing and Phasing of the CFTR Gene
    Poster: TLA & Long Read Sequencing: Efficient Targeted Sequencing and Phasing of the CFTR Gene
  • Application Note Pacific Biosciences - Targeted Sequencing and Chromosomal Haplotype Assembly Using Cergentis TLA Technology with PacBio SMRT Sequencing.
    Application Note Pacific Biosciences - Targeted Sequencing and Chromosomal Haplotype Assembly Using Cergentis TLA Technology with PacBio SMRT Sequencing.
  • Application note on the TLA protocol for isolated genomic DNA.
    Application note on the TLA protocol for isolated genomic DNA.
  • Publications
  • ZNF462 and KLF12 are disrupted by a de novo translocation in a patient with syndromic intellectual disability and autism spectrum disorder

    Cosemans N, et al.  (2018)                                                                                                                             

    University Hospital Leuven, Rady Children's Hospital & Leuven Autism Research 

  • Remapping of the belted phenotype in cattle on BTA3 identifies a multiplication event as the candidate causal mutation

    Rothammer S et al. (2018)                                                                                                                             

    LMU Munich & The L.K. Ernst Institute of Animal Husbandry           

  • Sensitive Monogenic Noninvasive Prenatal Diagnosis by Targeted Haplotyping

    Vermeulen C et al. (2017)                                                                                                                             

    Cergentis, Hubrecht Institute-KNAW, Kariminejad-Najmabadi Pathology & Genetics Center, National and Kapodistrian University of Athens, Sara Medical Genetics Lab, Shahid Beheshti University of Medical Sciences, University Medical Center Utrecht & University of Patras

  • Mapping the genomic landscape of inherited retinal disease genes prioritizes genes prone to coding and noncoding copy-number variations

    Van Schil et al. (2017)                                                                                                                             

    Ghent University, Harvard Medical School, Seattle Children's Research Institute, The Children's Hospital of Philadelphia, University of Cologne, University of Tuebingen, University of Washington School of Medicine

  • Targeted Locus Amplification and Next-Generation Sequencing

    Hottentot QP et al.  (2016)                                                                                                                             

    Cergentis & Leiden University Medical Center

  • Novel microdeletions on chromosome 14q32.2 suggest a potential role for non-coding RNAs in Kagami-Ogata syndrome

    Van der Werf IM et al.  (2016)                                                                                                                             

    Ghent University Hospital, Greenwood Genetic Center, Heinrich-Heine University, University Antwerpen & University Duisburg-Essen

  • mTORC1 Inhibition Corrects Neurodevelopmental and Synaptic Alterations in a Human Stem Cell Model of Tuberous Sclerosis

    Costa V et al.  (2016)                                                                                                                             

    Roche, Pvalue Research SRL, University of Basel, University Hospital Basel & University of Zurich

  • Haplotype-resolved genome sequencing: experimental methods and applications

    Snyder MW et al.  (2015)                                                                                                                             

    University of Washington, Oregon Health & Sciences University & University of Michigan

  • Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping

    De Vree PJP et al.  (2014)                                                                                                                             

    Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB

  • Webinars
  • Applications and principles of TLA-based complete gene sequencing and haplotyping

     

  • Phasing information of 307 SNVs across a 711 kb region spanning the entire CFTR gene and surrounding sequences. 
  • An overview of circular TLA template and generated linear TLA amplicons. Since both will consist of sequences originating from different positions within the same allele, paired end and long read sequencing of TLA amplicons enables haplotyping across large physical distances.
  • Phasing information of 307 SNVs across a 711 kb region spanning the entire CFTR gene and surrounding sequences. 
  • An overview of circular TLA template and generated linear TLA amplicons. Since both will consist of sequences originating from different positions within the same allele, paired end and long read sequencing of TLA amplicons enables haplotyping across large physical distances.

Compare technologies

See how TLA compares to other conventional approaches such as WGS and FISH.

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For Research Use Only.Not for use in diagnostic procedures.