Whereas conventional approaches for targeted sequencing are hypothesis-driven, largely miss intronic genetic variation and will fail to identify a large share of structural variants, Cergentis' TLA technology enables hypothesis-neutral, complete sequencing of any genomic locus using a single primer pair. TLA thereby enables the identification of all genetic variations in loci of interest.
The TLA technology is highly flexible. Any genomic region of interest can be sequenced by simply using one primer pair complementary to a short locus-specific sequence.
In addition, TLA enables haplotyping across large physical distances, both with short- and long-read sequencing technologies.*
* Cergentis does not currently offer this as a routine service. For more information please contact sales.
- Application notes
- Poster: TLA & Long Read Sequencing: Efficient Targeted Sequencing and Phasing of the CFTR Gene
- Application Note Pacific Biosciences - Targeted Sequencing and Chromosomal Haplotype Assembly Using Cergentis TLA Technology with PacBio SMRT Sequencing.
- Application note on the TLA protocol for isolated genomic DNA.
Cosemans N, et al. (2018)
University Hospital Leuven, Rady Children's Hospital & Leuven Autism Research
Rothammer S et al. (2018)
LMU Munich & The L.K. Ernst Institute of Animal Husbandry
Vermeulen C et al. (2017)
Cergentis, Hubrecht Institute-KNAW, Kariminejad-Najmabadi Pathology & Genetics Center, National and Kapodistrian University of Athens, Sara Medical Genetics Lab, Shahid Beheshti University of Medical Sciences, University Medical Center Utrecht & University of Patras
Van Schil et al. (2017)
Ghent University, Harvard Medical School, Seattle Children's Research Institute, The Children's Hospital of Philadelphia, University of Cologne, University of Tuebingen, University of Washington School of Medicine
Hottentot QP et al. (2016)
Cergentis & Leiden University Medical Center
Van der Werf IM et al. (2016)
Ghent University Hospital, Greenwood Genetic Center, Heinrich-Heine University, University Antwerpen & University Duisburg-Essen
Costa V et al. (2016)
Roche, Pvalue Research SRL, University of Basel, University Hospital Basel & University of Zurich
Snyder MW et al. (2015)
University of Washington, Oregon Health & Sciences University & University of Michigan
De Vree PJP et al. (2014)
Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB
Applications and principles of TLA-based complete gene sequencing and haplotyping