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Cell Line Development

Cell lines such as Chinese Hamster Ovary (CHO) cells are used in the development and production of therapeutic proteins. Ensuring correct genetic engineering is important in various stages of cell line development.

 

Cergentis' Targeted Locus Amplification (TLA) technology empowers:

  • The optimisation and validation of new cell line generation technologies
  • Clone selection
  • Clonality and genetic stability analyses
  • Application notes
  • Publications
  • Webinars

 

TLA analyses are used to identify:

  • The genomic position(s) of transgene integration site(s).
  • Single nucleotide variants in the transgene sequence.
  • Structural changes in the transgene sequence and
    integration site(s).
  • Genetic alterations resulting from targeted (trans)gene editing.

 

Cergentis supports many leading pharmaceutical companies in (CHO) cell line development and quality control of genetically engineered cells. 

 

Review an example report

  • Example of an NGS coverage profile across a transgene. A large part of the transgene has been sequenced with > 1000x coverage (i.e. with at least 1000 NGS reads). Y-axis shows NGS coverage, cut-off at 1000x. X-axis shows the position within the transgene sequence.
  • Example of a table with identified single nucleotide variants and indels in a transgene sequence.
  • Examples of whole-genome integration site analyses in the CHO genome. The top 50 scaffolds with NGS coverage are shown. The identified TLA coverage peaks, indicative of an integration event, are clearly visible. The left plot shows a sample with a single integration site in scaffold 1. The plot on the right shows a sample with 2 integrations: 1 in scaffold 8, the other in scaffold 28.
  • Example of an NGS coverage profile across a transgene. A large part of the transgene has been sequenced with > 1000x coverage (i.e. with at least 1000 NGS reads). Y-axis shows NGS coverage, cut-off at 1000x. X-axis shows the position within the transgene sequence.
  • Example of a table with identified single nucleotide variants and indels in a transgene sequence.
  • Examples of whole-genome integration site analyses in the CHO genome. The top 50 scaffolds with NGS coverage are shown. The identified TLA coverage peaks, indicative of an integration event, are clearly visible. The left plot shows a sample with a single integration site in scaffold 1. The plot on the right shows a sample with 2 integrations: 1 in scaffold 8, the other in scaffold 28.

TLA identifies

  • Transgene integration site(s)
  • SNVs in transgene sequence
  • Structural changes in transgene and integration site(s)
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