Genetically Engineered Models
Gene editing techniques, such as CRISPR/Cas9, ZFN, and TALENs, have redefined the boundaries of biological research and revolutionized animal models for understanding human diseases. In spite of these targeted techniques, unpredictable results can still occur, including:
- Off-target or multiple integrations
- Undesired / backbone sequence integration
- Structural variants in transgene or surrounding host genome
Therefore, characterizing gene editing outcomes is critical for:
- Assessing engineering efficacy and safety
- Improving genotype-phenotype correlation
- Facilitating proper selection of founders for experiments and publication
- Optimizing breeding and intercrossing
"TLA has become an indispensable tool to characterize transgene insertion events and complex gene targeting approaches.”
Unmatched genetic insights
Our proprietary TLA-based solutions can robustly detect all genetic variation in and around the gene of interest. We offer unrivalled genetic insights in a cost-attractive and time-effective alternative to conventional QC approaches, such as FISH or inverse PCR, as illustrated in this comparison table.
Comprehensive proprietary solutions tailored to your specific research needs
- Mapping exact transgene insertion sites
N/A
- Characterisation of (large) structural variations at integration sites in the host genome
- Complete targeted (trans)gene sequencing
- Detections of sequence variants and structural changes within the transgene
- (Transgene) copy number estimation
- Assessing the quality and efficiency of gene editing
- Confirming on-target genetic alterations
- Assess heterogeneity of genome-edited pools of cells
- QC large integrated vectors and their insertion sites (e.g. BAC, YAC)
- Identity assay for extremely homologous vectors
- Vector sequencing
- Genotyping strategy development
- QC of new transfection/transduction gene editing techniques
- Clone selection
- (MCB) Cell bank characterisation & clonality assessment
- Genetic stability analysis
Genetically engineered (animal) models
Cell line development
Gene therapy & upstream (USP) viral producer cell bank
Cergentis offers a range of services to characterize your transgenic model:
Transgenic models
- Identify transgene integration site(s)
- Detect structural changes in the host DNA at the transgene integration site(s)
- Sequence entire transgene to detect single nucleotide variants or structural changes
- Estimate transgene copy number
- Analyze samples containing multiple different integrated vectors
- Sequence large transgenes (e.g. BAC)
- Sequence plasmid or vector sequencing
- Genotyping primer design
Download example reports
Your go-to partner for complete sequencing of genetically engineered models
Our assays are routinely used to characterize transgenic, viral or BAC integrations in animals and cell lines and are considered the gold standard by industry and academia alike.
Organisms analysed by TLA
mosquito
fruit fly
spider
zebrafish
mouse
rat
hamster
chicken
rabbit
goat
pig
cattle
human
Your one-stop shop for genetic testing
- Transposon-mediated integrations
- PiggyBac
- Sleeping Beauty transposon
- CRISPR
- Viral integrations
- AAV
- Lentiviral integration events
- Retroviral
- HIV.HBV
- Etc.
- Random integration events
- Single or multiple vectors
- Targeted integrations via homologous recombination
- Targeted knock-outs
- Large vector (BAC constructs)
- Landing pad methods
- using Fip-FRT/Cre-LoxP
- CHO (Chinese hamster ovaries)
- Human cell lines
- HEK293
- hiPS
- ES
- T-cells
- Etc.
- Primary cells
Boasting over 50 peer-reviewed scientific publications
Our TLA-based assays have been featured in over 50 peer-reviewed publications, helping researchers across the world with their genetic QC challenges.
Click here to visit our publications page.
We are a globally recognized and trusted partner for genetic QC testing
Experience how easy our ordering process is.
