Genetically Engineered Models

Genetically Engineered Models

Gene editing techniques, such as CRISPR/Cas9, ZFN, and TALENs, have redefined the boundaries of biological research and revolutionized animal models for understanding human diseases. In spite of these targeted techniques, unpredictable results can still occur, including:

  • Off-target or multiple integrations
  • Undesired / backbone sequence integration
  • Structural variants in transgene or surrounding host genome

Therefore, characterizing gene editing outcomes is critical for:

  • Assessing engineering efficacy and safety
  • Improving genotype-phenotype correlation
  • Facilitating proper selection of founders for experiments and publication
  • Optimizing breeding and intercrossing

"TLA has become an indispensable tool to characterize transgene insertion events and complex gene targeting approaches.”

Taconic
Dr. Jochen Welcker - Director Molecular Biology & Scientific Development

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Unmatched genetic insights

Our proprietary TLA-based solutions can robustly detect all genetic variation in and around the gene of interest. We offer unrivalled genetic insights in a cost-attractive and time-effective alternative to conventional QC approaches, such as FISH or inverse PCR, as illustrated in this comparison table.

Comprehensive proprietary solutions tailored to your specific research needs

Genetic characterisationIndustry Application
  • Mapping exact transgene insertion sites
    • N/A
  • Characterisation of (large) structural variations at integration sites in the host genome
    • N/A
  • Complete targeted (trans)gene sequencing
    • N/A
  • Detections of sequence variants and structural changes within the transgene
    • N/A
  • (Transgene) copy number estimation
    • N/A
  • Assessing the quality and efficiency of gene editing
    • N/A
  • Confirming on-target genetic alterations
    • N/A
  • Assess heterogeneity of genome-edited pools of cells
    • N/A
  • QC large integrated vectors and their insertion sites (e.g. BAC, YAC)
    • N/A
  • Identity assay for extremely homologous vectors
    • N/A
  • Vector sequencing
    • N/A
  • Genotyping strategy development
    • N/A
  • QC of new transfection/transduction gene editing techniques
    • N/A
  • Clone selection
    • N/A
  • (MCB) Cell bank characterisation & clonality assessment
    • N/A
  • Genetic stability analysis
    • N/A
Select your application area
  • Genetically engineered (animal) models
  • Cell line development
  • Gene therapy & upstream (USP) viral producer cell bank
  • Cell therapy & downstream (DSP)

 

Cergentis offers a range of services to characterize your transgenic model:

Transgenic models

  • Identify transgene integration site(s)
  • Detect structural changes in the host DNA at the transgene integration site(s)
  • Sequence entire transgene to detect single nucleotide variants or structural changes
  • Estimate transgene copy number
  • Analyze samples containing multiple different integrated vectors
  • Sequence large transgenes (e.g. BAC)
  • Sequence plasmid or vector sequencing
  • Genotyping primer design

Download example reports

Your go-to partner for complete sequencing of genetically engineered models

Our assays are routinely used to characterize transgenic, viral or BAC integrations in animals and cell lines and are considered the gold standard by industry and academia alike.

 

Organisms analysed by TLA

  • mosquito
  • fruit fly
  • spider
  • zebrafish
  • mouse
  • rat
  • hamster
  • chicken
  • rabbit
  • goat
  • pig
  • cattle
  • human

Your one-stop shop for genetic testing

Multiple (gene editing) tools
  • Transposon-mediated integrations
    • PiggyBac
    • Sleeping Beauty transposon
  • CRISPR
  • Viral integrations
    • AAV
    • Lentiviral integration events
    • Retroviral
    • HIV.HBV
    • Etc.
  • Random integration events
  • Single or multiple vectors
  • Targeted integrations via homologous recombination
  • Targeted knock-outs
  • Large vector (BAC constructs)
  • Landing pad methods
    • using Fip-FRT/Cre-LoxP
Cell lines
  • CHO (Chinese hamster ovaries)
  • Human cell lines
    • HEK293
    • hiPS
    • ES
    • T-cells
    • Etc.
  • Primary cells

Boasting over 50 peer-reviewed scientific publications

Our TLA-based assays have been featured in over 50 peer-reviewed publications, helping researchers across the world with their genetic QC challenges.
Click here to visit our publications page.

We are a globally recognized and trusted partner for genetic QC testing

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For Research Use Only.Not for use in diagnostic procedures.
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