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Genome Editing

Genome editing techniques including CRISPR-Cas9 enable the generation of both small and large genetic alterations, holding promise in both fundamental and applied research.

 

Thorough, hypothesis-free interrogation of on-site genetic alterations is essential in determining the efficacy and safety of your genome editing efforts.

 

TLA analyses, using primers in proximity to a targeted site, can be used to determine which structural changes have occurred in your target locus by generating >100kb sequencing coverage surrounding the target site. 

 

In addition, TLA analyses can, using transgene-specific primer pairs, resolve the exact sequence of desired and undesired integration events of any transgene sequence.

 

Our experienced team of scientists has seen a wide range of genome-edited models and resolved complex genomic rearrangements in thousands of samples, making Cergentis your dependable partner for the complete sequencing of your edited loci.

 

  • Application notes
  • Publications
  • Webinars
  • A.) Strategy to evaluate clones in which 4 target sites (green arrows) were used to knock-out 2 genes located on 2 chromosomes. Double headed arrows show position of the TLA primer sets used. B.) A total of 8 different alleles were identified in which insertions, translocations and duplications were identified.
  • Schematic depictions of the NGS coverage profiles resulting from different rearrangements that can result from gene editing. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the targeted site is shown below each coverage plot.
  • Overview of applying TLA at different stages of generating genome-edited cells and organisms.
  • A.) Strategy to evaluate clones in which 4 target sites (green arrows) were used to knock-out 2 genes located on 2 chromosomes. Double headed arrows show position of the TLA primer sets used. B.) A total of 8 different alleles were identified in which insertions, translocations and duplications were identified.
  • Schematic depictions of the NGS coverage profiles resulting from different rearrangements that can result from gene editing. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the targeted site is shown below each coverage plot.
  • Overview of applying TLA at different stages of generating genome-edited cells and organisms.

TLA enables you to determine

  • The sequence of a complete locus
  • Genome editing outcomes
  • (Complex) structural rearrangements
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