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Genome Editing

Genome editing techniques such as CRISPR-Cas9 enable the generation of both small and large genetic alterations. 

 

TLA analyses, using primers in proximity to a targeted site, can be used to determine which sequence or structural changes have occurred in targeted sites. 

 

In addition, TLA analyses can, using transgene-specific primer pairs, be used to determine the exact sequence of desired and undesired integration events of any transgene sequence. 

  • Application notes
  • Publications
  • Webinars

LIVE WEBINAR

  • 19 February 2020
  • TLA: Improved genetic characterisation and clonality assessment for (CHO) cell line development
  • For more information and registration, click below:
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  • A.) Strategy to evaluate clones in which 4 target sites (green arrows) were used to knock-out 2 genes located on 2 chromosomes. Double headed arrows show position of the TLA primer sets used. B.) A total of 8 different alleles were identified in which insertions, translocations and duplications were identified.
  • Schematic depictions of the NGS coverage profiles resulting from different rearrangements that can result from gene editing. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the targeted site is shown below each coverage plot.
  • Overview of applying TLA at different stages of generating genome-edited cells and organisms.
  • A.) Strategy to evaluate clones in which 4 target sites (green arrows) were used to knock-out 2 genes located on 2 chromosomes. Double headed arrows show position of the TLA primer sets used. B.) A total of 8 different alleles were identified in which insertions, translocations and duplications were identified.
  • Schematic depictions of the NGS coverage profiles resulting from different rearrangements that can result from gene editing. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the targeted site is shown below each coverage plot.
  • Overview of applying TLA at different stages of generating genome-edited cells and organisms.

TLA enables you to determine

  • The sequence of a complete locus
  • Genome editing outcomes
  • (Complex) structural rearrangements
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