Genome Editing

Genome editing techniques including CRISPR-Cas9 enable the generation of both small and large genetic alterations, holding promise in both fundamental and applied research.

Thorough, hypothesis-free interrogation of on-site genetic alterations is essential in determining the efficacy and safety of your genome editing efforts.

TLA analyses, using primers in proximity to a targeted site, can be used to determine which structural changes have occurred in your target locus by generating >100kb sequencing coverage surrounding the target site. 

In addition, TLA analyses can, using transgene-specific primer pairs, resolve the exact sequence of desired and undesired integration events of any transgene sequence.

Our experienced team of scientists has seen a wide range of genome-edited models and resolved complex genomic rearrangements in thousands of samples, making Cergentis your dependable partner for the complete sequencing of your edited loci.

• Application notes
• Publications
• Webinars

• Application notes
• 
  Appnote: TLA for complete locus sequencing and quality control of targeted genome editing

  Read

• Publications
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  A genome-wide CRISPR/Cas phenotypic screen for modulators of DUX4 cytotoxicity reveals screen complications

  Ashoti A et al. (2020)                                                                                                                             

  Hubrecht Institute, Harvard University, Broad Institute of MIT and Harvard, Erasmus MC, LUMC & Single Cell Discoveries  

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  Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus

  Goldstein JM et al. (2019)                                                                                                            

  Harvard University, Harvard Medical School, Harvard Stem Cell Institute & Joslin Diabetes Center

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  Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches

  Leidy-Davis T et al. (2018)                                                                                                            

  A*STAR, Duke-NUS Medical School, Duke University Medical Center, The Jackson Laboratory, Nanyang Technological University, National Cancer Centre Singapore & Singapore General Hospital

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  Applications of CRISPR genome editing technology in drug target identification and validation

  Lu Q et al. (2017)                                                                                                                             

  GlaxoSmithKline & Wellcome Trust Sanger Institute

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  Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

  Eyquem J et al. (2017)                                                                                                            

  Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute

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  Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

  Renaud J-B et al. (2016)                                                                                                            

  Amagen, CHU de Nantes, genOway & Museum National d'Histoire Naturelle

• Webinars
• Unbiased and complete sequencing of transgenic and genome edited loci using TLA