Genome editing techniques including CRISPR-Cas9 enable the generation of both small and large genetic alterations, holding promise in both fundamental and applied research.
Thorough, hypothesis-free interrogation of on-site genetic alterations is essential in determining the efficacy and safety of your genome editing efforts.
TLA analyses, using primers in proximity to a targeted site, can be used to determine which structural changes have occurred in your target locus by generating >100kb sequencing coverage surrounding the target site.
In addition, TLA analyses can, using transgene-specific primer pairs, resolve the exact sequence of desired and undesired integration events of any transgene sequence.
Our experienced team of scientists has seen a wide range of genome-edited models and resolved complex genomic rearrangements in thousands of samples, making Cergentis your dependable partner for the complete sequencing of your edited loci.