Genome editing techniques including CRISPR-Cas9 enable the generation of both small and large genetic alterations, holding promise in both fundamental and applied research.
Thorough, hypothesis-free interrogation of on-site genetic alterations is essential in determining the efficacy and safety of your genome editing efforts.
TLA analyses, using primers in proximity to a targeted site, can be used to determine which structural changes have occurred in your target locus by generating >100kb sequencing coverage surrounding the target site.
In addition, TLA analyses can, using transgene-specific primer pairs, resolve the exact sequence of desired and undesired integration events of any transgene sequence.
Our experienced team of scientists has seen a wide range of genome-edited models and resolved complex genomic rearrangements in thousands of samples, making Cergentis your dependable partner for the complete sequencing of your edited loci.
- Application notes
- Appnote: TLA for complete locus sequencing and quality control of targeted genome editing
Ashoti A et al. (2020)
Hubrecht Institute, Harvard University, Broad Institute of MIT and Harvard, Erasmus MC, LUMC & Single Cell Discoveries
Goldstein JM et al. (2019)
Harvard University, Harvard Medical School, Harvard Stem Cell Institute & Joslin Diabetes Center
Leidy-Davis T et al. (2018)
A*STAR, Duke-NUS Medical School, Duke University Medical Center, The Jackson Laboratory, Nanyang Technological University, National Cancer Centre Singapore & Singapore General Hospital
Lu Q et al. (2017)
GlaxoSmithKline & Wellcome Trust Sanger Institute
Eyquem J et al. (2017)
Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute
Renaud J-B et al. (2016)
Amagen, CHU de Nantes, genOway & Museum National d'Histoire Naturelle
Unbiased and complete sequencing of transgenic and genome edited loci using TLA