Genome editing techniques such as CRISPR-Cas9 enable the generation of both small and large genetic alterations.
TLA analyses, using primers in proximity to a targeted site, can be used to determine which sequence or structural changes have occurred in targeted sites.
In addition, TLA analyses can, using transgene-specific primer pairs, be used to determine the exact sequence of desired and undesired integration events of any transgene sequence.
- Application notes
- Appnote: TLA for complete locus sequencing and quality control of targeted genome editing
Goldstein JM et al. (2019)
Harvard University, Harvard Medical School, Harvard Stem Cell Institute & Joslin Diabetes Center
Leidy-Davis T et al. (2018)
A*STAR, Duke-NUS Medical School, Duke University Medical Center, The Jackson Laboratory, Nanyang Technological University, National Cancer Centre Singapore & Singapore General Hospital
Lu Q et al. (2017)
GlaxoSmithKline & Wellcome Trust Sanger Institute
Eyquem J et al. (2017)
Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute
Renaud J-B et al. (2016)
Amagen, CHU de Nantes, genOway & Museum National d'Histoire Naturelle
TLA technology & complete NGS of transgenes, integration sites and gene editing events