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Transgenic Models

Transgenic cell lines, animals and plants are widely used in scientific research and in the biotech industry.

 

The suitability of transgenic lines often depends on the location of the transgene integration site(s) and the sequence and structural integrity of integrated transgene sequences. The latter determines whether a transgene results in desired or aberrant gene expression.

 

Transgene integrations can have important phenotypic consequences, for example by affecting endogenous gene function. The identification of integration sites can also be required for the optimisation of breeding programs or be a requirement for regulatory approval.

 

TLA analyses enable the sequencing of integration sites and integrated sequences in a wide variety of applications.

 

Applications include: 

  • Transgene & integration site sequencing in cell lines and animal models
  • Viral genome and integration site sequencing in a wide variety of species
  • Application notes
  • Publications
  • Webinars

 

TLA analyses identify:

  • The genomic position(s) of integration site(s)
  • Breakpoint sequences between the transgene (vector) and genome for genotyping
  • Single nucleotide variants in the transgene (vector)/viral sequence
  • Structural changes in the transgene (vector)/viral sequence
  • TLA-based transgene sequencing. Using one TLA primer pair complementary to a sequence unique to the transgene, complete sequence information is generated across the transgene and its integration site(s).
  • Example of a table with identified single nucleotide variants and indels in a transgene sequence.
  • Example of a whole-genome coverage plot showing eight transgene integration sites in the pig genome. The identified integration sites are encircled.
  • Schematic depictions of the NGS coverage profiles of different rearrangements resulting from transgene integration. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the integrated transgene sequence is shown below each coverage plot. Red arrows indicate the position of the breakpoint sequences.
  • TLA-based transgene sequencing. Using one TLA primer pair complementary to a sequence unique to the transgene, complete sequence information is generated across the transgene and its integration site(s).
  • Example of a table with identified single nucleotide variants and indels in a transgene sequence.
  • Example of a whole-genome coverage plot showing eight transgene integration sites in the pig genome. The identified integration sites are encircled.
  • Schematic depictions of the NGS coverage profiles of different rearrangements resulting from transgene integration. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the integrated transgene sequence is shown below each coverage plot. Red arrows indicate the position of the breakpoint sequences.

TLA can

  • Identify transgene integration site(s)
  • Identify SNVs and SVs in transgene sequence
  • Assess structural changes in integration site(s)
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