Transgenic Models

Transgenic Models

Transgenic cell lines and animals are widely used in fundamental and applied research.

The suitability of transgenic lines often depends on the location of the transgene integration site(s) and the sequence and structural integrity of integrated transgene sequences. The latter determines whether a transgene results in desired or aberrant gene expression.

Transgene integrations can have important phenotypic consequences, for example by affecting endogenous gene function. The identification of integration sites can also be required for the optimization of breeding programs or a requirement for regulatory approval.

TLA analyses enable the sequencing of integration sites and integrated sequences in a wide variety of applications. Today, TLA is considered the gold standard for the characterization of genetically engineered cells and animals by industry and academia alike.

Applications include: 

  • Transgene & integration site sequencing in cell lines and animal models
  • BAC integration site sequencing
  • Viral genome and integration site sequencing in a wide variety of species

 

Organisms analyzed by TLA

Cergentis routinely characterizes transgenic, viral or BAC integrations in animals and cell lines, thereby completely characterizing their sequence, integrity and integration site(s) generating >100kb of sequencing coverage in a single TLA experiment.

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Our customer base consists of the majority of leading pharmaceutical companies and research institutes worldwide, making Cergentis your go-to partner for the complete sequencing of your animal models.

Download an example report for transgenic mouse models generated through homologous recombination here. Or download our example report of a mouse model generated through random integration here.

In addition to TLA, Cergentis offers a range of services to further characterize your transgenic and genome-edited model, including:

 

For more information or questions, please reach out to our sales team.

TLA-based transgene sequencing. Using one TLA primer pair complementary to a sequence unique to the transgene, complete sequence information is generated across the transgene and its integration site(s).
Example of a table with identified single nucleotide variants and indels in a transgene sequence.
Example of a whole-genome coverage plot showing eight transgene integration sites in the pig genome. The identified integration sites are encircled.
Schematic depictions of the NGS coverage profiles of different rearrangements resulting from transgene integration. The coverage profiles shown result from mapping the generated reads to the host genome. A map of the host genome with the integrated transgene sequence is shown below each coverage plot. Red arrows indicate the position of the breakpoint sequences.
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