Publications

The TLA technology was first published in Nature Biotechnology in October 2014. Various customers and partners have since published their use of TLA in a variety of applications.  

Please select an application to review the related publications:

• Cell Line Development
• 
  Multiplexed clonality verification of cell lines for protein biologic production

  O'Brien SA et al.  (2020)                                                                                                                             

  Bayer HealthCare & University of Minnesota

• 
  Enhanced CHO clone screening: Applying Targeted Locus Amplification and Next-Generation Sequencing technologies during cell line development

  Aeschlimann SH et al. (2019)                                                                                                                             

  Novartis Institutes for BioMedical Research & Cergentis  

• 
  Analytical assessment of clonal derivation of eukaryotic/CHO cell populations

  Aebischer-Gumy C et al. (2019)                                                                                                                             

  Glenmark Pharmaceuticals & Thomas A. Little Consulting

• 
  Antibody expression stability in CHO clonally-derived cell lines and their subclones: Role of methylation in phenotypic and epigenetic heterogeneity

  Patel NA et al. (2018)                                                                                                                                                                    

  Seattle Genetics

• 
  Isolation and characterization of a monoclonal antibody containing an extra heavy-light chain Fab arm

  Boyd D et al. (2018)                                                                                                                                                                

  Gilead Sciences

• 
  Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange

  Baumann M et al. (2017)                                                                                                                                    

  Austrian Centre of Industrial Biotechnology (ACIB), MilliporeSigma & University of Natural Resources and Life Sciences (BOKU)

• 
  New Mammalian Expression Systems                                                                                          

  Zhu J & Hatton D (2017)                                                                                                                                                                

  MedImmune

• 
  Targeted sequencing for comprehensive genetic characterization of a recombinant CHO cell line

  Mickus B (Presentation - 2016)                                                                                                                                         

  Gilead Sciences

• 
  Deep sequencing reveals different compositions of mRNA transcribed from the F8 gene in a panel of FVIII-producing CHO cell lines

  Kaas CS et al. (2015)                                                                                                                             

  Novo Nordisk & Technical University of Denmark

• Cell and Gene Therapy
• 
  TEG001 insert integrity from vector producer cells until medicinal product

  Straetemans T et al. (2019)                                                                                                            

  University Medical Center Utrecht, Utrecht University & Cergentis

• 
  Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionality

  Sachdeva M et al. (2019)                                                                                                                                                           

  Cellectis

• 
  Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

  Eyquem J et al. (2017)                                                                                                            

  Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute

• Clinical Genetics
• 
  ZNF462 and KLF12 are disrupted by a de novo translocation in a patient with syndromic intellectual disability and autism spectrum disorder

  Cosemans N, et al.  (2018)                                                                                                                             

  University Hospital Leuven, Rady Children's Hospital & Leuven Autism Research 

• 
  Remapping of the belted phenotype in cattle on BTA3 identifies a multiplication event as the candidate causal mutation

  Rothammer S et al. (2018)                                                                                                                             

  LMU Munich & The L.K. Ernst Institute of Animal Husbandry           

• 
  Sensitive Monogenic Noninvasive Prenatal Diagnosis by Targeted Haplotyping

  Vermeulen C et al. (2017)                                                                                                                             

  Cergentis, Hubrecht Institute-KNAW, Kariminejad-Najmabadi Pathology & Genetics Center, National and Kapodistrian University of Athens, Sara Medical Genetics Lab, Shahid Beheshti University of Medical Sciences, University Medical Center Utrecht & University of Patras

• 
  Mapping the genomic landscape of inherited retinal disease genes prioritizes genes prone to coding and noncoding copy-number variations

  Van Schil et al. (2017)                                                                                                                             

  Ghent University, Harvard Medical School, Seattle Children's Research Institute, The Children's Hospital of Philadelphia, University of Cologne, University of Tuebingen, University of Washington School of Medicine

• 
  Targeted Locus Amplification and Next-Generation Sequencing

  Hottentot QP et al.  (2016)                                                                                                                             

  Cergentis & Leiden University Medical Center

• 
  Novel microdeletions on chromosome 14q32.2 suggest a potential role for non-coding RNAs in Kagami-Ogata syndrome

  Van der Werf IM et al.  (2016)                                                                                                                             

  Ghent University Hospital, Greenwood Genetic Center, Heinrich-Heine University, University Antwerpen & University Duisburg-Essen

• 
  mTORC1 Inhibition Corrects Neurodevelopmental and Synaptic Alterations in a Human Stem Cell Model of Tuberous Sclerosis

  Costa V et al.  (2016)                                                                                                                             

  Roche, Pvalue Research SRL, University of Basel, University Hospital Basel & University of Zurich

• 
  Haplotype-resolved genome sequencing: experimental methods and applications

  Snyder MW et al.  (2015)                                                                                                                             

  University of Washington, Oregon Health & Sciences University & University of Michigan

• 
  Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping

  De Vree PJP et al.  (2014)                                                                                                                             

  Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB

• Genome Editing
• 
  Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus

  Goldstein JM et al. (2019)                                                                                                            

  Harvard University, Harvard Medical School, Harvard Stem Cell Institute & Joslin Diabetes Center

• 
  Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches

  Leidy-Davis T et al. (2018)                                                                                                            

  A*STAR, Duke-NUS Medical School, Duke University Medical Center, The Jackson Laboratory, Nanyang Technological University, National Cancer Centre Singapore & Singapore General Hospital

• 
  Applications of CRISPR genome editing technology in drug target identification and validation

  Lu Q et al. (2017)                                                                                                                             

  GlaxoSmithKline & Wellcome Trust Sanger Institute

• 
  Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

  Eyquem J et al. (2017)                                                                                                            

  Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute

• 
  Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

  Renaud J-B et al. (2016)                                                                                                            

  Amagen, CHU de Nantes, genOway & Museum National d'Histoire Naturelle

• Leukaemia
• 
  Genetic Screening Test to Detect Translocations in Acute Leukemias by Use of Targeted Locus Amplification

  Alimohamed MZ et al. (2018)                                                                                                            

  Cergentis & University of Groningen

• 
  Tyrosine kinase fusion genes in pediatric BCR-ABL1-like acute lymphoblastic leukemia

  Boer JM et al. (2016)                                                                                                            

  COALL, DCOG, Erasmus Medical Center, Princess Maxima Center for Pediatric Oncology, Radboud University Medical Center & University of Amsterdam

• 
  Cergentis TLA and NGS in Pediatric ALL.

  Splinter E et al. (Poster - 2016)                                                                                                            

  Cergentis, DCOG, Erasmus Medical Center, Princess Maxima Center for Pediatric Oncology & Radboud University Medical Center

• 
  Targeted Locus Amplification & Next Generation Sequencing for the Detection of Recurrent and Novel Gene Fusions for Improved Treatment Decisions in Pediatric Acute Lymphoblastic Leukemia

  Kuiper RP et al. (2015)                                                                                                            

  Cergentis, DCOG, Erasmus Medical Center, Radboud University Medical Center

• 
  Identification of Candidate Oncogenes and Chromosomal Breakpoint Sequencing By Targeted Locus Amplification in T-Cell Acute Lymphoblastic Leukemia

  Vroegindeweij EM et al. (2015)                                                                                                            

  Cergentis, Erasmus Medical Center, Ghent University, KU Leuven & Princess Maxima Center for Pediatric Oncology

• Protocols
• 
  Targeted Locus Amplification and Next-Generation Sequencing

  Hottentot QP et al. (2016)                                                                                                            

  Cergentis & Leiden University Medical Center

• Solid Tumours
• 
  Targeted Locus Amplification (TLA): A Novel Next Generation Sequencing (NGS) Technology to Detect New Molecular Markers and Monitoring Minimal Residual Disease (MRD) in Mantle Cell and Follicular Lymphoma

  Genuardi E et al. (2017)                                                                                                            

  A.O.U. Citta della Salute e della Scienza di Torino, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Cergentis, Clinica Humanitas/Gavazzeni, University of Modena e Reggio Emilia & University of Torino

• 
  Personalized Medicine in Lymphoma: Tailoring Treatment According to Minimal Residual Disease

  Dogliotti I & Ferrero S (2017)                                                                                                            

  A.O.U. Citta della Salute e della Scienza di Torino & University of Torino

• 
  Spectrum of genetic variants of BRCA1 and BRCA2 in a German single center study

  Meisel C et al. (2017)                                                                                                            

  DKFZ, Medical Genetic Center Munich, National Center for Tumor Diseases (NCT), TU Dresden, University Kiel

• 
  Activating ESR1 Mutations Differentially Impact the Efficacy of ER Antagonists

  Toy W et al. (2016)                                                                                                                             

  AstraZeneca, Memorial Sloan Kettering Cancer Center, University of Chicago & University of Illinois at Urbana Champaign

• 
  Mechanisms of Therapy Resistance in Patient-Derived Xenograft Models of BRCA1-Deficient Breast Cancer

  Ter Brugge P et al. (2016)                                                                                                            

  Bellvitge Biomedical Research Institute Barcelona, Curie Institute, ICREA, Institute of Cancer Research London, The Netherlands Cancer Institute, University of Barcelona & University Medical Center Utrecht

• 
  Sequencing Structural Variants in Cancer for Precision Therapeutics

  Macintyre G et al. (2016)                                                                                                            

  University of Cambridge & VU University Medical Center

• 
  Guidelines for cytogenetic investigations in tumours

  Hastings RJ et al. (2015)                                                                                                            

  Charles University Prague, CHUV, Great Ormond St Hospital, Gustave Roussy, Hospital del Mar, Institute of Genomic Medicine, Oslo University Hospital, Ospedale di Circolo-Polo Universitario, Our Lady's Children's Hospital, Oxford University Hospitals NHS Trust, Radboud University Medical Center, St James's Hospital, University of Cagliari, University of Groningen, University Hospital Ghent, UZ Leuven, VU University Medical Center

• 
  Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping

  De Vree PJP et al.  (2014)                                                                                                                             

  Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB

• Transgenic Models
• 
  Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy

  Cañes L et al. (2020)                                                                                                            

  Instituto de Investigaciones Biomédicas de Barcelona (IIBB-CSIC), Institut de Recerca del Hospital de la Santa Creu i Sant Pau, Hospital Universitario Marqués de Valdecilla, Universidad de Zaragoza & Paris Institute of Technology for Life Food and Environmental Sciences

• 
  A mouse model for vitamin D-induced human cathelicidin antimicrobial peptide gene expression

  Lowry MB, et al.  (2019)                                                                                                                             

  Oregon State University, Oregon Health, Science University, University of Nebraska Medical Center, University of California San Diego, UCLA & National University of Singapore 

• 
  Safety evaluation of conditionally immortalized cells for renal replacement therapy

  Mihajlovic M et al. (2019)                                                                                                                             

  Utrecht University & Radboud University Medical Center

• 
  Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis

  Godet I et al. (2019)                                                                                                                                             

  The Johns Hopkins University

• 
  Introduction of the TLA sequencing analyses for a better identification of the transgene integrations, to select porcine founder animals for breeding

  Perota A et al. (Conference Paper - 2019)                                                                                                                 

  Avantea & KU Leuven

• 
  A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression

  Bryda EC et al.  (2019)                                                                                                                                                             

  University of Missouri

• 
  Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice

  Mathews S et al. (2019)                                                                                                                             

  Central Institute for Experimental Animals Japan & University Of Nebraska Medical Center

• 
  Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis

  Goodwin LO et al. (2019)                                                                                                                             

  The Jackson Laboratory & Cergentis

• 
  The integration site of the APP transgene in the J20 mouse model of Alzheimer’s disease

  Tosh JL et al.  (2018)                                                                                                                             

  University College London & University of California San Francisco

• 
  Mapping Transgene Insertion Sites Reveals Complex Interactions Between Mouse Transgenes And Neighboring Endogenous Genes

  Laboulaye MA et al. (2018)                                                                                                                                                

  Harvard University

• 
  Chromosome structural anomalies due to aberrant spindle forces exerted at gene editing sites in meiosis

  Manil-Segalen M et al. (2018)                                                                                                                             

  Collège de France, Institut Curie & University of Southampton

• 
  Lineage-specific functions of TET1 in the postimplantation mouse embryo

  Khoueiry R et al. (2017)                                                                                                                                    

  KU Leuven, La Jolla Institute for Allergy and Immunology & VIB

• 
  Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

  Eyquem J et al. (2017)                                                                                                            

  Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute

• 
  Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification

  Cain-Hom C et al. (2016)                                                                                                                                 

  Genentech & Cergentis

• 
  Activating ESR1 Mutations Differentially Impact the Efficacy of ER Antagonists

  Toy W et al. (2016)                                                                                                                             

  AstraZeneca, Memorial Sloan Kettering Cancer Center, University of Chicago & University of Illinois at Urbana Champaign

• 
  A transgenic mouse expressing CHMP2B intron 5 mutant in neurons develops histological and behavioural features of amyotrophic lateral sclerosis and frontotemporal dementia

  Vernay A et al. (2016)                                                                                                            

  Hôpitaux civils and CMRR, Université Joseph Fourier & Université de Strasbourg

• 
  TransgeneOmics - A transgenic platform for protein localization based function exploration

  Hasse A et al. (2016)                                                                                                            

  Max Planck Institute of Molecular Cell Biology & Genetics

• 
  Transgenic animals and genetic engineering techniques                                    

  Menoret S et al. (2015)                                                                                                            

  CHU de Nantes & SFR François Bonamy​

• 
  Flagellin-induced NLRC4 phosphorylation primes the inflammasome for activation by NAIP5

  Matusiak M et al. (2015)                                                                                                            

  Ghent University, Institut Pasteur de Lille, St. Jude Children's Research Hospital & VIB

• 
  Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping

  De Vree PJP et al.  (2014)                                                                                                                             

  Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB