The TLA technology was first published in Nature Biotechnology in October 2014. Various customers and partners have since published their use of TLA in a variety of applications.
Please select an application to review the related publications:
Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing
Stadermann A et al. (2021)
Boehringer Ingelheim & Genedata
Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing.
Jo S et al. (2021)
Cellectis, CEBR & Institut Paoli Calmettes
Development of recombinase-based targeted integration systems for production of exogenous proteins using transposon-mediated landing pads
Reese NB & Ku S (2021)
Just - Evotec Biologics
Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs
Kemter E et al. (2021)
Ludwig Maximilian University of Munich, German Center for Diabetes Research, Technische Universität Dresden, Helmholtz Center Munich, Max Planck Institute for Medical Research, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Max Planck Institute of Molecular Cell Biology and Genetics & University Hospital Carl Gustav Carus
Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy
Müller TR et al. (2021)
Technical University of Munich, Helmholtz Centre for Infection Research, German Center for Infection Research (DZIF) & Medical University of Vienna
Minimal residual disease (MRD) detection in acute lymphoblastic leukaemia based on fusion genes and genomic deletions: towards MRD for all
Kuiper RP et al. (2021)
Princess Máxima Center for Pediatric Oncology, Erasmus MC & Cergentis
Breakpoint characterization of a rare alpha 0 -thalassemia deletion using targeted locus amplification on genomic DNA
Hottentot QP et al. (2021)
Leiden University Medical Center (LUMC)
Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing
Allahyar A et al. (2021)
Hubrecht Institute, Cergentis, UMC Utrecht, LabPON, Leiden UMC, UMC Groningen & Amsterdam UMC
Targeted Locus Amplification to Detect Molecular Markers in Mantle Cell and Follicular Lymphoma
Genuardi E et al. (2021)
University of Torino, European Myeloma Network Onlus, Humanitas/Gavazzeni Clinic, AOU Città della Salute e della Scienza di Torino, University of Modena and Reggio Emilia, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo & Cergentis
CRISPR-Targeted CAR Gene Insertion Using Cas9/RNP and AAV6 Enhances Anti-AML Activity of Primary NK Cells
Kararoud MN et al. (2021)
Nationwide Children’s Hospital, The Ohio State University, University of Minnesota & Thomas Jefferson University
When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping
Sailer S et al. (2021)
Medical University of Innsbruck, Goethe-University & University of Oxford
Homozygosity of BACHD rats not only causes strong behavioral deficits in young female rats but also a reduced breeding success
Kurat S et al. (2021)
QPS Austria
CHOK1SV GS‐KO® SSI Expression System: A combination of the Fer1L4 locus and glutamine synthetase selection
Feary M et al. (2021)
Lonza & Pfizer
Characterization of the adiponectin promoter + Cre recombinase insertion in the Tg(Adipoq-cre)1Evdr mouse by targeted locus amplification and droplet digital PCR
Wong MA et al. (2021)
NIH
Predicting favorable landing pads for targeted integrations in Chinese hamster ovary cell lines by learning stability characteristics from random transgene integrations
Dhiman H, Campbell M, Melcher M, Smith KD & Borth N. (2020)
Janssen R&D, University of Natural Resources and Life Sciences & Austrian Centre of Industrial Biotechnology
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Rapid lentiviral vector producer cell line generation using a single DNA construct
Chen YH et al. (2020)
GlaxoSmithKline
A genome-wide CRISPR/Cas phenotypic screen for modulators of DUX4 cytotoxicity reveals screen complications
Ashoti A et al. (2020)
Hubrecht Institute, Harvard University, Broad Institute of MIT and Harvard, Erasmus MC, LUMC & Single Cell Discoveries
Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region
Bae DH et al. (2020)
FDA & NIH
Transgene integration causes RARB downregulation in homozygous Tg4-42 mice
Hinteregger B et al. (2020)
QPS Austria GmbH, Medical University of Graz, BioTechMed-Graz, Vienna University of Technology & University Medical Center Göttingen
Regulatory Dynamics of Tet1 and Oct4 Resolve Stages of Global DNA Demethylation and Transcriptomic Changes in Reprogramming
Bartoccetti M et al. (2020)
KU Leuven
Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy
Cañes L et al. (2020)
Instituto de Investigaciones Biomédicas de Barcelona (IIBB-CSIC), Institut de Recerca del Hospital de la Santa Creu i Sant Pau, Hospital Universitario Marqués de Valdecilla, Universidad de Zaragoza & Paris Institute of Technology for Life Food and Environmental Sciences
TEG001 insert integrity from vector producer cells until medicinal product
Straetemans T et al. (2019)
University Medical Center Utrecht, Utrecht University & Cergentis
Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus
Goldstein JM et al. (2019)
Harvard University, Harvard Medical School, Harvard Stem Cell Institute & Joslin Diabetes Center
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A mouse model for vitamin D-induced human cathelicidin antimicrobial peptide gene expression
Lowry MB, et al. (2019)
Oregon State University, Oregon Health, Science University, University of Nebraska Medical Center, University of California San Diego, UCLA & National University of Singapore
Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionality
Sachdeva M et al. (2019)
Cellectis
Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis
Godet I et al. (2019)
The Johns Hopkins University
PRMT6 Promotes Lung Tumor Progression via the Alternate Activation of Tumor-Associated Macrophages
Avasarala S et al. (2020)
University of Illinois at Chicago, Rush University Medical Center, The University of Texas MD Anderson Cancer Center, University of Science and Technology of China, University of Illinois Cancer Center & Jesse Brown VA Medical Center
Introduction of the TLA sequencing analyses for a better identification of the transgene integrations, to select porcine founder animals for breeding
Perota A et al. (Conference Paper - 2019)
Avantea & KU Leuven
A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
Bryda EC et al. (2019)
University of Missouri
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Safety evaluation of conditionally immortalized cells for renal replacement therapy
Mihajlovic M et al. (2019)
Utrecht University & Radboud University Medical Center
High-throughput identification of human SNPs affecting regulatory element activity
van Arensbergen J et al. (2019)
Netherlands Cancer Institute, Columbia University Medical Center, Radboud University Nijmegen, University of Cambridge, University of Groningen & University of Tartu
Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice
Mathews S et al. (2019)
Central Institute for Experimental Animals Japan & University Of Nebraska Medical Center
Enhanced CHO clone screening: Applying Targeted Locus Amplification and Next-Generation Sequencing technologies during cell line development
Aeschlimann SH et al. (2019)
Novartis Institutes for BioMedical Research & Cergentis
Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis
Goodwin LO et al. (2019)
The Jackson Laboratory & Cergentis
Analytical assessment of clonal derivation of eukaryotic/CHO cell populations
Aebischer-Gumy C et al. (2019)
Glenmark Pharmaceuticals & Thomas A. Little Consulting
Mapping Transgene Insertion Sites Reveals Complex Interactions Between Mouse Transgenes And Neighboring Endogenous Genes
Laboulaye MA et al. (2018)
Harvard University
The integration site of the APP transgene in the J20 mouse model of Alzheimer’s disease
Tosh JL et al. (2018)
University College London & University of California San Francisco
Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches
Leidy-Davis T et al. (2018)
A*STAR, Duke-NUS Medical School, Duke University Medical Center, The Jackson Laboratory, Nanyang Technological University, National Cancer Centre Singapore & Singapore General Hospital
Chromosome structural anomalies due to aberrant spindle forces exerted at gene editing sites in meiosis
Manil-Segalen M et al. (2018)
Collège de France, Institut Curie & University of Southampton
Reprogramming human T cell function and specificity with non-viral genome targeting
Roth TL et al. (2018)
UC San Francisco, UC Los Angeles, UC Berkeley, Yale School of Medicine, Stanford University, University of Chicago, Takara Bio USA, National Cancer Institute, University of Nebraska Medical Center, The Children’s Hospital of Philadelphia, The Perelman School of Medicine at the University of Pennsylvania & Chan Zuckerberg Biohub
Remapping of the belted phenotype in cattle on BTA3 identifies a multiplication event as the candidate causal mutation
Rothammer S et al. (2018)
LMU Munich & The L.K. Ernst Institute of Animal Husbandry
Genetic Screening Test to Detect Translocations in Acute Leukemias by Use of Targeted Locus Amplification
Alimohamed MZ et al. (2018)
Cergentis & University of Groningen
Antibody expression stability in CHO clonally-derived cell lines and their subclones: Role of methylation in phenotypic and epigenetic heterogeneity
Patel NA et al. (2018)
Seattle Genetics
Isolation and characterization of a monoclonal antibody containing an extra heavy-light chain Fab arm
Boyd D et al. (2018)
Gilead Sciences
ZNF462 and KLF12 are disrupted by a de novo translocation in a patient with syndromic intellectual disability and autism spectrum disorder
Cosemans N, et al. (2018)
University Hospital Leuven, Rady Children's Hospital & Leuven Autism Research
Targeted Locus Amplification (TLA): A Novel Next Generation Sequencing (NGS) Technology to Detect New Molecular Markers and Monitoring Minimal Residual Disease (MRD) in Mantle Cell and Follicular Lymphoma
Genuardi E et al. (2017)
A.O.U. Citta della Salute e della Scienza di Torino, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Cergentis, Clinica Humanitas/Gavazzeni, University of Modena e Reggio Emilia & University of Torino
Sensitive Monogenic Noninvasive Prenatal Diagnosis by Targeted Haplotyping
Vermeulen C et al. (2017)
Cergentis, Hubrecht Institute-KNAW, Kariminejad-Najmabadi Pathology & Genetics Center, National and Kapodistrian University of Athens, Sara Medical Genetics Lab, Shahid Beheshti University of Medical Sciences, University Medical Center Utrecht & University of Patras
Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping
De Vree PJP et al. (2014)
Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB
Mapping the genomic landscape of inherited retinal disease genes prioritizes genes prone to coding and noncoding copy-number variations
Van Schil et al. (2017)
Ghent University, Harvard Medical School, Seattle Children's Research Institute, The Children's Hospital of Philadelphia, University of Cologne, University of Tuebingen, University of Washington School of Medicine
Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange
Baumann M et al. (2017)
Austrian Centre of Industrial Biotechnology (ACIB), MilliporeSigma & University of Natural Resources and Life Sciences (BOKU)
New Mammalian Expression Systems
Zhu J & Hatton D (2017)
MedImmune
Lineage-specific functions of TET1 in the postimplantation mouse embryo
Khoueiry R et al. (2017)
KU Leuven, La Jolla Institute for Allergy and Immunology & VIB
Personalized Medicine in Lymphoma: Tailoring Treatment According to Minimal Residual Disease
Dogliotti I & Ferrero S (2017)
A.O.U. Citta della Salute e della Scienza di Torino & University of Torino
Applications of CRISPR genome editing technology in drug target identification and validation
Lu Q et al. (2017)
GlaxoSmithKline & Wellcome Trust Sanger Institute
Spectrum of genetic variants of BRCA1 and BRCA2 in a German single center study
Meisel C et al. (2017)
DKFZ, Medical Genetic Center Munich, National Center for Tumor Diseases (NCT), TU Dresden, University Kiel
Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection
Eyquem J et al. (2017)
Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute
Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection
Eyquem J et al. (2017)
Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute
Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection
Eyquem J et al. (2017)
Memorial Sloan Kettering Cancer Center & Sloan Kettering Institute
Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification
Cain-Hom C et al. (2016)
Genentech & Cergentis
Activating ESR1 Mutations Differentially Impact the Efficacy of ER Antagonists
Toy W et al. (2016)
AstraZeneca, Memorial Sloan Kettering Cancer Center, University of Chicago & University of Illinois at Urbana Champaign
Activating ESR1 Mutations Differentially Impact the Efficacy of ER Antagonists
Toy W et al. (2016)
AstraZeneca, Memorial Sloan Kettering Cancer Center, University of Chicago & University of Illinois at Urbana Champaign
Tyrosine kinase fusion genes in pediatric BCR-ABL1-like acute lymphoblastic leukemia
Boer JM et al. (2016)
COALL, DCOG, Erasmus Medical Center, Princess Maxima Center for Pediatric Oncology, Radboud University Medical Center & University of Amsterdam
Targeted Locus Amplification and Next-Generation Sequencing
Hottentot QP et al. (2016)
Cergentis & Leiden University Medical Center
A transgenic mouse expressing CHMP2B intron 5 mutant in neurons develops histological and behavioural features of amyotrophic lateral sclerosis and frontotemporal dementia
Vernay A et al. (2016)
Hôpitaux civils and CMRR, Université Joseph Fourier & Université de Strasbourg
Sequencing Structural Variants in Cancer for Precision Therapeutics
Macintyre G et al. (2016)
University of Cambridge & VU University Medical Center
Novel microdeletions on chromosome 14q32.2 suggest a potential role for non-coding RNAs in Kagami-Ogata syndrome
Van der Werf IM et al. (2016)
Ghent University Hospital, Greenwood Genetic Center, Heinrich-Heine University, University Antwerpen & University Duisburg-Essen
Mechanisms of Therapy Resistance in Patient-Derived Xenograft Models of BRCA1-Deficient Breast Cancer
Ter Brugge P et al. (2016)
Bellvitge Biomedical Research Institute Barcelona, Curie Institute, ICREA, Institute of Cancer Research London, The Netherlands Cancer Institute, University of Barcelona & University Medical Center Utrecht
Cergentis TLA and NGS in Pediatric ALL.
Splinter E et al. (Poster - 2016)
Cergentis, DCOG, Erasmus Medical Center, Princess Maxima Center for Pediatric Oncology & Radboud University Medical Center
Targeted sequencing for comprehensive genetic characterization of a recombinant CHO cell line
Mickus B (Presentation - 2016)
Gilead Sciences
mTORC1 Inhibition Corrects Neurodevelopmental and Synaptic Alterations in a Human Stem Cell Model of Tuberous Sclerosis
Costa V et al. (2016)
Roche, Pvalue Research SRL, University of Basel, University Hospital Basel & University of Zurich
TransgeneOmics - A transgenic platform for protein localization based function exploration
Hasse A et al. (2016)
Max Planck Institute of Molecular Cell Biology & Genetics
Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases
Renaud J-B et al. (2016)
Amagen, CHU de Nantes, genOway & Museum National d'Histoire Naturelle
Targeted Locus Amplification & Next Generation Sequencing for the Detection of Recurrent and Novel Gene Fusions for Improved Treatment Decisions in Pediatric Acute Lymphoblastic Leukemia
Kuiper RP et al. (2015)
Cergentis, DCOG, Erasmus Medical Center, Radboud University Medical Center
Identification of Candidate Oncogenes and Chromosomal Breakpoint Sequencing By Targeted Locus Amplification in T-Cell Acute Lymphoblastic Leukemia
Vroegindeweij EM et al. (2015)
Cergentis, Erasmus Medical Center, Ghent University, KU Leuven & Princess Maxima Center for Pediatric Oncology
Transgenic animals and genetic engineering techniques
Menoret S et al. (2015)
CHU de Nantes & SFR François Bonamy
Deep sequencing reveals different compositions of mRNA transcribed from the F8 gene in a panel of FVIII-producing CHO cell lines
Kaas CS et al. (2015)
Novo Nordisk & Technical University of Denmark
Haplotype-resolved genome sequencing: experimental methods and applications
Snyder MW et al. (2015)
University of Washington, Oregon Health & Sciences University & University of Michigan
Guidelines for cytogenetic investigations in tumours
Hastings RJ et al. (2015)
Charles University Prague, CHUV, Great Ormond St Hospital, Gustave Roussy, Hospital del Mar, Institute of Genomic Medicine, Oslo University Hospital, Ospedale di Circolo-Polo Universitario, Our Lady's Children's Hospital, Oxford University Hospitals NHS Trust, Radboud University Medical Center, St James's Hospital, University of Cagliari, University of Groningen, University Hospital Ghent, UZ Leuven, VU University Medical Center
Flagellin-induced NLRC4 phosphorylation primes the inflammasome for activation by NAIP5
Matusiak M et al. (2015)
Ghent University, Institut Pasteur de Lille, St. Jude Children's Research Hospital & VIB
Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping
De Vree PJP et al. (2014)
Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB
Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping
De Vree PJP et al. (2014)
Cergentis, Erasmus Medical Center, Ghent University, Hubrecht Institute-KNAW, Leiden University Medical Center, The Netherlands Cancer Institute, Radboud University Medical Center, University of Amsterdam, University of Groningen, University Medical Center Utrecht & VIB
Targeted Locus Amplification and Next-Generation Sequencing
Hottentot QP et al. (2016)
Cergentis & Leiden University Medical Center
