TLA for FFPE Tumour Samples

The Targeted Locus Amplification (TLA) technology uses crosslinking and the physical proximity of nucleotides within a locus of interest as the basis of selection. As such, TLA presents particular advantages in crosslinked FFPE samples.

In the TLA technology for FFPE tumour samples, crosslinked DNA fragments are religated to generate TLA template: combinations of religated DNA fragments that prior to formaldehyde crosslinking occurred in extreme physical proximity to each other and therefore originated from the same genomic locus. TLA template is used as input material for enrichment and targeted sequencing. In this manner, TLA in combination with conventional capture or linear PCR-based enrichment, enables the enrichment of all sequences that originated from the same locus.

As a result, the TLA FFPE protocol uniquely enables the targeted complete sequencing and the detection of all single nucleotide and structural variants in genes of interest.