Menu

Comparing TLA to conventional techniques

We display in the table below the various advantages that TLA technology presents over conventional approaches. Comparison tables have been created for 3 specific applications, namely: Transgene & Integration Site, Cancer, and Gene Sequencing & Haplotyping. For each application, you will be able to evaluate the capabilities of our TLA analysis against popular techniques, based on several criteria. Through this simple yet interactive TLA one-pager, you will be able to access to more in-depth explanations by hovering on top of cells that contain an "i” icon.

 

1. Select the application you are interested in

2. Compare our TLA technology with any of the suggested alternatives by (de)selecting the method(s) you are interested in

 

 

Rotate your screen for a better view of the table.
  • WGS
  • PCR-based/Capture
  • qPCR/ddPCR
  • Southern blot
  • RNA Sequencing
  • FISH
  • Transgene & Integration Site
  • Cancer
  • Gene Sequencing & Haplotyping
  • Criteria
  • Integration sites (TG)
  • Structural variation
  • Co-integrations
  • Transgene Sequence Variants
  • SNVs
  • Indels
  • Rearrangements
  • Concatemers
  • Copy number
  • TLA combined with NGS
  • i
    TLA can provide a copy number estimation based on 3 variables:
    - the number of integration sites
    - the number of transgene-transgene fusions
    - the ratio of the sequencing coverage on the transgene-side and genome-side of the integration site

    TLA only provides information on cells with integrated TG
  • WGS
  • i
    Only based on the identification of breakpoint fusion reads
  • i
    Only at high coverage
  • i
    Only at high coverage
  • i
    Cannot reliably detect structural variations in and around (trans)gene of interest
  • i
    Only at high coverage
  • i
    Assesses copy numbers in population of cells. It enables the assessment of TG sequencing coverage vs. the coverage across the host genome
  • PCR-based/Capture
  • i
    Only confirmation of known integration sites (not novel ones)
  • i
    Only short indels
  • i
    Only confirmation of known TG-TG fusions (not novel ones)
  • i
    Only confirmation of known TG-TG fusions (not novel ones)
  • i
    Known breakpoint marking integration site can be evaluated to determine the frequency of the integration site in a population
  • qPCR/ddPCR
  • i
    Only confirmation of known TG-TG fusions (not novel ones)
  • i
    Only confirmation of known TG-TG fusions (not novel ones)
  • Southern blot
  • i
    Structural variations can be identified/detected, but sequence information will not be generated
  • FISH
  • Criteria
  • Complete gene sequencing
  • Single Nucleotide Variants
  • Structural Variants
  • Indels
  • Gene fusions
  • Complex rearrangements
  • Copy number variation (CNV)
  • TLA combined with NGS
  • i
    TLA analyses can be used to quantify the relative abundance of different genomic loci
  • WGS
  • i
    Only at high coverage
  • i
    Only at high coverage
  • i
    Only at high coverage
  • i
    Cannot reliably detect structural variations in and around (trans)gene of interest
  • PCR-based/Capture
  • i
    Limited ability to sequence long genomic sequences.
    Does require a priori knowledge and therefore, will only be able to detect short indels and/or the presence of known mutations only
  • i
    Only in targeted loci
  • i
    Only short indels
  • i
    Only confirmation of known rearrangements (not novel ones)
  • RNA Sequencing
  • i
    Only in coding sequences of expressed genes
  • i
    Only in coding sequences of expressed genes
  • i
    Only in coding sequences of expressed genes
  • FISH
  • Criteria
  • Complete gene sequencing
  • Single Nucleotide Variants
  • Structural Variants
  • Indels
  • Gene fusions
  • Complex rearrangements
  • Copy number variation (CNV)
  • Haplotyping
  • TLA combined with NGS
  • i
    TLA can provide a copy number estimation based on 3 variables:
    - the number of integration sites
    - the number of transgene-transgene fusions
    - the ratio of the sequencing coverage on the transgene-side and genome-side of the integration site

    TLA only provides information on cells with integrated TG
  • i
    Because of more sequencing depth, it is possible to determine on which locus the variance of interest is present
  • WGS
  • i
    Only at high coverage
  • i
    Only at high coverage
  • i
    Only at high coverage
  • i
    Cannot reliably detect structural variations in and around (trans)gene of interest
  • i
    Assesses copy numbers in population of cells. It enables the assessment of TG sequencing coverage vs. the coverage across the host genome
  • PCR-based/Capture
  • i
    Limited ability to sequence long genomic sequences.
    Does require a priori knowledge and therefore, will only be able to detect short indels and/or the presence of known mutations only.
  • i
    Only short indels
  • i
    Only confirmation of known rearrangements (not novel ones)
© 2012-2019 Cergentis B.V. All rights reserved.
For Research Use Only.Not for use in diagnostic procedures.
We use cookies for website analysis and marketing. By visiting and using our website you accept these cookies.
ACCEPT